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. 2009 Nov 3;18(2):295–306. doi: 10.1038/mt.2009.252

Figure 1.

Figure 1

(a) Schematic representation of the microbubble delivery of Ad-GFP complexes and ultrasound (US) release in a tumor target site of the mouse. (b) Western blot analysis of Ad-GFP/microbubble–transduced DU-145 tumor xenografts. Immunoblot showing the expression levels of green fluorescent protein (GFP) in DU-145 cells following ultrasound-targeted microbubble/Ad transduction of GFP at 96 hours. Only the tumor on the right flank was sonoporated for 10 minutes resulting in the delivery and expression of GFP. The left tumor, heart, lung, liver, and kidney were negative for GFP expression. Purified glutathione-S-transferase–GFP was used as a positive control. Protein gel loading was normalized using β-actin as a control. (c) Ultrasound imaging and US contrast enhancement of in vivo transduced DU-145 tumor xenografts. B-mode US imaging of a tumor before MB contrast agent injection. (d) B-mode US imaging of the same tumor depicted in c following injection of microbubbles/Ad-GFP complexes. MBs cavitation within the targeted tumor dramatically enhances the tumor image within the US field of view. Ad, adenovirus, MB, microbubbles.