Effects of ACs on 3H-TdR incorporation by PBT cells stimulated with immobilized anti-CD3 mAb (A and D), SEB (B), or phytohemagglutinin (C). PBT cells were cultured in the absence (No) or presence of indicated percentages of C-ACs, Tat-ACs, Lps-ACs, or Tc-ACs. The 3H-TdR incorporation is expressed as the mean cpm of triplicate cultures and the vertical bars represent standard derivation of the mean (n = 3). Concentrations of either rTat protein or Lps used to treat ACs are indicated inside parentheses otherwise 1 μM of Tat protein is used. A representative experiment of five (A), three (B), or two (C and D) performed is shown.