Figure 6.

PI (A) and Hoechst (B) staining of apoptotic cell death in PBT cells stimulated with immobilized anti-CD3 mAb in the presence of ACs. (A) PBT cells were stimulated for 4 days at 37°C in the presence of either 8% or 28% of Tat-ACs, Tc-ACs or C-ACs, after which the cells were stained by PI and DNA contents were examined by flow cytometry. The percentages of cells identified as apoptotic cells (subG₁ cell population) are indicated. (B) PBT cells were stimulated for 4 days at 37°C in the presence of 28% of indicated ACs, after which the cells were stained with FITC-conjugated anti-CD2 mAb plus phycoerythrin-conjugated anti-CD5 mAb, or with FITC-conjugated anti-CD14 mAb alone, followed by Hoechst staining. The percentages denote Ho^bright cells within CD2⁺CD5⁺ or CD14⁺ cell population.