Table 3.
WT DmdNK | R4.V3 | R4.V3-[85] | R4.V3-[172] | |
---|---|---|---|---|
Thr85Met | … Glu172Val | Thr85Met … | ||
Glu172Val | Tyr179Phe | Tyr179Phe | ||
Tyr179Phe | His193Tyr | His193Tyr | ||
His193Tyr | ||||
T | 4813 | 0.1 (−48,000) | 1.4 (−3,400) | 326 (−14) |
dC | 5850 | 0.36 (−16,000) | 0.39 (−15,000) | 2925 (−2) |
dA | 161 | nd | nd | 57 (−2.5) |
dG | 28 | nd | nd | 6 (−4.5) |
ddT | 4.6 | 3 (−1.5) | 28 (+6) | 2.6 (−1.8) |
fddT | 1.3 | 1.9 (+1.5) | 0.4 (−3) | 7.5 (+6) |
The kcat/KM values (× 103 s−1 M−1) of the parental enzyme, as well as evolved variants are listed. The fold change relative to DmdNK are shown in parentheses. nd = not detectable. Data were adapted from ref. [42].