. Author manuscript; available in PMC: 2010 Nov 1.

Published in final edited form as: Chimia (Aarau). 2009 Nov 1;63(11):737–744. doi: 10.2533/chimia.2009.737

Table 3.

Catalytic performance of ddT-kinases

	WT DmdNK	R4.V3	R4.V3-[85]	R4.V3-[172]
		Thr85Met	… Glu172Val	Thr85Met …
		Glu172Val	Tyr179Phe	Tyr179Phe
		Tyr179Phe	His193Tyr	His193Tyr
		His193Tyr
T	4813	0.1 (−48,000)	1.4 (−3,400)	326 (−14)
dC	5850	0.36 (−16,000)	0.39 (−15,000)	2925 (−2)
dA	161	nd	nd	57 (−2.5)
dG	28	nd	nd	6 (−4.5)
ddT	4.6	3 (−1.5)	28 (+6)	2.6 (−1.8)
fddT	1.3	1.9 (+1.5)	0.4 (−3)	7.5 (+6)

The k_cat/K_M values (× 10³ s⁻¹ M⁻¹) of the parental enzyme, as well as evolved variants are listed. The fold change relative to DmdNK are shown in parentheses. nd = not detectable. Data were adapted from ref. [42].