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Influence of the beam energy and the distance between the scintillator and the sample resulting in the attenuation of phase contrast or absorption contrast and the impact on the visual detail. Non-decalcified histological cartilage sample embedded in epoxy resin mounted on a polymer microscope slide. Sample to scintillator distances greater than 2 cm increase the visual detail. The higher beam energy of (b) 15 keV compared with (a) 10 keV slightly increases the visual detail.
