Figure 1.

Expression of the 2C TCR as a covalently linked αβ heterodimer from Drosophila melanogaster cells. (A) Schematic diagram of the 2C expression construct design (see Materials and Methods). (B) The expressed 2C is a covalent heterodimer. On the left, SDS/PAGE analysis of Ni²⁺-agarose purified 2C. On the right, 2D nonequilibrium, pH-gradient electrophoresis. Both gels are Coomassie-stained. (C) The recombinant TCR 2C binds with high affinity to the Fab of the anti-idiotypic mAb 1B2 (21) using SPR on a BIAcore 2000 machine.