Figure 3.

Rescue of HIV-based lentiviral vector transduction by LEDGF_325–530 hybrids. WT and LEDGF BC cells are used as controls. (a) Relative luciferase activity (RLU/µg protein) following HIV-based vector transduction (LV CMV eGFP-T2A-fLuc). Data are compiled at least six independent experiments and are expressed as percentages relative to LEDGF BC cells (mean ± SD). Complemented B5 and D11 cells resulted in similar results (Supplementary Figure S3). (b) Vector integration measured by Q-PCR in WT cells and LEDGF BC cells and LEDGF_325–530 fusions, data are represented as mean ± SD. CBX1, heterochromatin protein 1β (formerly HP1β); CMV, cytomegalovirus; eGFP, enhanced green fluorescent protein; H1, histone H1; HIV, human immunodeficiency virus; IN, integrase; KD, knockdown; LEDGF, lens epithelium–derived growth factor; LV, lentivirus; Q-PCR, quantitative PCR; RLU, relative light units; WT, wild type.