Figure 4.

Rescue of EIAV-based vector transduction by LEDGF hybrids. LEDGF_325–530 fusions are tested for their ability to rescue EIAV-eGFP transduction. (a) eGFP fluorescence was evaluated by fluorescence-activated cell-sorting analysis. Data are compiled at least six independent experiments and are expressed relative to LEDGF BC complemented cells (mean ± SD). (b) EIAV integration measured by Q-PCR. CBX1, heterochromatin protein 1β (formerly HP1β); eGFP, enhanced green fluorescent protein; EIAV, equine infectious anemia virus; H1, histone H1; KD, knockdown; LEDGF, lens epithelium–derived growth factor; LV, lentivirus; Q-PCR, quantitative PCR; WT, wild type.