Figure 6.

Effect of retargeting by CBX1-LEDGF_325–530 on transgene expression over time. WT and KD cells, together with LEDGF BC and eGFP-LEDGF_325–530 cells were used as controls. All cells were transduced with an HIV-based vector carrying both eGFP and fLuc as reporter genes (LV CMV eGFP-T2A-fLuc) as in Supplementary Figure S3. Reporter activity was determined at the indicated time point following HIV-based vector (LV CMV eGFP-T2A-fLuc) transduction (days post-transduction). (a) Overall eGFP fluorescence over time is calculated as MFI × % gated cells and displayed as mean ± SD (n = 6). (b) Relative luciferase activity (RLU/µg protein) over time; data are expressed as mean ± SD (n = 6). CBX1, heterochromatin protein 1β (formerly HP1β); CMV, cytomegalovirus; eGFP, enhanced green fluorescent protein; fLuc, firefly luciferase; H1, histone H1; HIV, human immunodeficiency virus; KD, knockdown; LEDGF, lens epithelium–derived growth factor; MFI, mean fluorescence intensity; RLU, relative light units; WT, wild type.