Figure 5.

Visualization of compartments involved in intracellular association of the HEL-(46–61) determinant with A^k. RBL stably transfected with wild-type I-A^k and invariant chain were incubated for 6 h in medium containing BSA or HEL at 3 mg/ml, as indicated. Cells were washed, fixed, permeabilized, and stained with the indicated mAbs. C4H3 binding was revealed with FITC (green); LAMP-1 or transferrin receptor was revealed with Cy3 (red). Images were acquired with a ×63 objective lens. Excitation was at 468–514 nm and emission was collected at 540 ± 15 nm for FITC and 580 ± 15 nm for Cy3.