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Noninvasive imaging of the wash-in process of Evans blue in cortical vasculature. (a) and (b): The PA images obtained before and after the dye injection. (c), (d), (e): PA image differences relative to (a) at time 32 s, 56 s, and 80 s, respectively. All these images were obtained at 620-nm wavelength. (f) The normalized value of the integral of the pixel values within the marked square area in (a) versus time. (Color online only.)
