Table 1.

Experimental sample size for confocal microscopy.

	Sample size definition		No. Images∕ plate	No. plates	Total No. of samples
Integrated fluorescence intensity	Treated	1 Sample=Average intensity of all cells per image	6	2	12 per cell line
	Untreated	1 Sample=Average intensity of all cells per image	6	2	12 per cell line
Spectrally resolved fluorescence	Treated	1 Sample=3 spectra averaged from a cluster of cells (3–5 cells) per image	6	2	12 per cell line
	Untreated	1 Sample=3 spectra averaged from a cluster of cells (3–5 cells) per image	1	1	1 per cell line