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. Author manuscript; available in PMC: 2010 Mar 16.
Published in final edited form as: Curr Biol. 2005 Apr 12;15(7):661–666. doi: 10.1016/j.cub.2005.02.051

Figure 2. Human APOBEC3G Cosediments with Ty1 VLPs.

Figure 2

(A) Western blot analysis of sucrose gradient fractions of cell lysates from strain JC3212 expressing APOBEC3G or no APOBEC with rabbit antiserum specific for Ty1 Gag (top) or anti-HA antibodies (bottom). For each strain, 10 ug of whole cell lysate (IN), 7 μl (1×) and 0.7 μl (0.1×) of the 45% fraction of the first sucrose gradient and 11.5 μl of the first and then every third fraction of the second 20% to 60% gradient (20%–60%) were analyzed.

(B) Similar to (A) except an spt3 strain expressing APOBEC3G was analyzed with anti-HA antibodies.