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. Author manuscript; available in PMC: 2010 Mar 16.
Published in final edited form as: Biochemistry. 2003 Jul 1;42(25):7682–7693. doi: 10.1021/bi034602h

Figure 4.

Figure 4

Steady-state parameters of Msh2-Msh6 ATPase. (A) Msh2-Msh6 ATPase varies with NaCl and yields kcat = 0.25–0.35 s−1 alone (□) and peak kcat = 0.65 and 0.55 s−1 near 150 and 170 mM NaCl concentrations with G:C (◇) and G:T DNA (●), respectively (30 °C). (B) ATP dependence of the steady-state activity yields kcat and Km values for Msh2-Msh6 in the absence and presence of various DNA substrates at 25 °C.