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. Author manuscript; available in PMC: 2010 Mar 16.
Published in final edited form as: Plant J. 2008 Jan 16;54(2):249–259. doi: 10.1111/j.1365-313X.2008.03410.x

Figure 2.

Figure 2

Cadmium exposure results in the appearance of phytochelatins (PCs) in the phloem sap of Brassica napus. Thiols from (a) control and plants exposed to 75 µm CdSO4 for (b) 1 day, (c) 1 week and (d) 2 weeks were labeled with monobromobimane, separated by reverse phase HPLC and detected by fluorescence. Thiols were identified with a mass spectrometer coupled to the HPLC. Phytochelatins appeared after 1 day of cadmium exposure and their concentrations increased with the time of exposure. Note the difference in the Y-axis scales in panels (c) and (d). The insets in (d) show the mass spectrum of the corresponding monobromobimane-labeled thiols. Bimane label accounts for the addition of 190 Da per thiol to the mass of the unlabeled compounds.