Figure 5.

FACS analysis of ERK dependency in mediating EGF‐induced delay in G₂/M progression. HeLa cells were synchronized at the G₁/S boundary and released back into the cell cycle. Cells were left untreated (control), treated with EGF (100 ng/mL) at 7 h after G₁/S release, treated with U0126 (10 µm) at 6.5 h after G₁/S release, or treated with EGF and U0126 together. Cells were harvested at the times indicated after release from G₁/S and the number of cells (Y axis) containing 2N or 4N DNA content (X axis) were determined by FACS.