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Expression of p21CIP1 is required for PMA‐induced G2‐phase arrest. HCT116 parental (a and c) or p21 CIP1 knockout cells (b and d) were synchronized at the G1/S‐phase boundary, treated in the absence (closed bars) or presence (open bars) of 0.1 µm PMA at 6 h after release, and harvested at the times indicated after G1/S release for FACS analysis of G2/M‐phase cells (a and b) or mitotic indices (c and d).
