Table 1. Peptide ELISA screening, quantitative mouse IgG ELISA, and manual SISCAPA results for ADAM17(VDN) mouse primary clone antibodies that were tested by manual SISCAPA.
Primary clone ID (mADAM17(VDN)-X) | A | B | C | D | E |
---|---|---|---|---|---|
Peptide-glutaraldehyde-BSA ELISA optical density (OD) | Free peptide ELISA OD | Supernatant antibody concentration (μg/mL) (n=2)a | Manual SISCAPA Volume of supernatant containing 1.65 mg antibody for coupling to 55 μL of beads (μL) | Manual SISCAPA Recovery efficiency (%) (n=1) | |
2 | 0.66 | 0.10 | 19.0 +/- 0.2 | 87 | 1 |
3 | 0.19 | 0.08 | 26.4 +/- 0.3 | 63 | 0 |
5 | 0.78 | 0.27 | 5.2 +/- 0.9* | 351 | 0 |
6 | 0.70 | 0.12 | 38.9 +/- 2.3 | 42 | 8 |
23 | 0.53 | 0.09 | 22.2 +/- 0.4 | 74 | 1 |
29 | 0.52 | 0.07 | 19.3 +/- 0.5 | 85 | 0 |
38 | 0.37 | 0.09 | 24.8 +/- 0.3 | 67 | 0 |
39 | 0.69 | 0.09 | 22.0 +/- 2.3 | 75 | 7 |
41 | 0.32 | 0.07 | 42.8 +/- 0.1 | 39 | 1 |
48 | 0.07 | 0.09 | 31.9 +/- 0.6 | 52 | 0 |
49 | 0.06 | 0.07 | 19.8 +/- 1.1 | 83 | 0 |
54 | 1.23 | 0.21 | 45.7 +/- 3.9* | 36 | 2 |
55 | 0.17 | 0.09 | 8.9 +/- 0.1 | 185 | 0 |
57 | 0.51 | 0.07 | 12.1 +/- 0.1 | 136 | 1 |
61 | 0.75 | 0.09 | 30.3 +/- 4.9* | 54 | 1 |
67 | 1.07 | 0.10 | 15.5 +/- 0.8* | 106 | 3 |
71 | 0.50 | 0.09 | 57.8 +/- 1.0 | 29 | 1 |
72 | 0.52 | 0.09 | 13.1 +/- 1.0 | 126 | 1 |
83 | 0.95 | 0.10 | 43.8 +/- 3.5* | 38 | 18 |
85 | 0.75 | 0.09 | 30.7 +/- 2.5 | 54 | 4 |
88 | 1.22 | 0.10 | 12.0 +/- 1.3* | 138 | 12 |
90 | 0.49 | 0.08 | 21.0 +/- 0.2 | 79 | 1 |
n=2 for most primary clone supernatants and the uncertainty given indicates the range of values. However, for the primary clone supernatants marked with an *, n=4 and the uncertainty given represents +/- one standard deviation.