Table 1. Peptide ELISA screening, quantitative mouse IgG ELISA, and manual SISCAPA results for ADAM17(VDN) mouse primary clone antibodies that were tested by manual SISCAPA.
Grey shaded fields indicate positive ELISA results (see Methods section).
| Primary clone ID (mADAM17(VDN)-X) | A | B | C | D | E |
|---|---|---|---|---|---|
| Peptide-glutaraldehyde-BSA ELISA optical density (OD) | Free peptide ELISA OD | Supernatant antibody concentration (μg/mL) (n=2)a | Manual SISCAPA Volume of supernatant containing 1.65 mg antibody for coupling to 55 μL of beads (μL) | Manual SISCAPA Recovery efficiency (%) (n=1) | |
| 2 | 0.66 | 0.10 | 19.0 +/- 0.2 | 87 | 1 |
| 3 | 0.19 | 0.08 | 26.4 +/- 0.3 | 63 | 0 |
| 5 | 0.78 | 0.27 | 5.2 +/- 0.9* | 351 | 0 |
| 6 | 0.70 | 0.12 | 38.9 +/- 2.3 | 42 | 8 |
| 23 | 0.53 | 0.09 | 22.2 +/- 0.4 | 74 | 1 |
| 29 | 0.52 | 0.07 | 19.3 +/- 0.5 | 85 | 0 |
| 38 | 0.37 | 0.09 | 24.8 +/- 0.3 | 67 | 0 |
| 39 | 0.69 | 0.09 | 22.0 +/- 2.3 | 75 | 7 |
| 41 | 0.32 | 0.07 | 42.8 +/- 0.1 | 39 | 1 |
| 48 | 0.07 | 0.09 | 31.9 +/- 0.6 | 52 | 0 |
| 49 | 0.06 | 0.07 | 19.8 +/- 1.1 | 83 | 0 |
| 54 | 1.23 | 0.21 | 45.7 +/- 3.9* | 36 | 2 |
| 55 | 0.17 | 0.09 | 8.9 +/- 0.1 | 185 | 0 |
| 57 | 0.51 | 0.07 | 12.1 +/- 0.1 | 136 | 1 |
| 61 | 0.75 | 0.09 | 30.3 +/- 4.9* | 54 | 1 |
| 67 | 1.07 | 0.10 | 15.5 +/- 0.8* | 106 | 3 |
| 71 | 0.50 | 0.09 | 57.8 +/- 1.0 | 29 | 1 |
| 72 | 0.52 | 0.09 | 13.1 +/- 1.0 | 126 | 1 |
| 83 | 0.95 | 0.10 | 43.8 +/- 3.5* | 38 | 18 |
| 85 | 0.75 | 0.09 | 30.7 +/- 2.5 | 54 | 4 |
| 88 | 1.22 | 0.10 | 12.0 +/- 1.3* | 138 | 12 |
| 90 | 0.49 | 0.08 | 21.0 +/- 0.2 | 79 | 1 |
a
n=2 for most primary clone supernatants and the uncertainty given indicates the range of values. However, for the primary clone supernatants marked with an *, n=4 and the uncertainty given represents +/- one standard deviation.