Fig 1.

In vivo AMSC phenotype. AMSCs were dendritic in shape and maintained intercellular contacts in situ (A). Cell viability, dendritic morphology and intercellular contacts were better visualized by staining with the Live and Dead assay (B). AMSCs did not express α-SMA (C) or desmin (D). In contrast, as a positive control, umbilical cord mesenchymal cells showed strong staining to both α-SMA and desmin (inset of C and D, respectively). However, all AMSCs expressed vimentin (E). Dot lines separate AM epithelium from AM stroma. Nuclear counterstaining was performed by DAPI (C,D) and PI (E), respectively. Bar represents 50 µm.