Fig 6.

Reversal of myofibroblasts by ASE was not associated with cell proliferation. Passage 3 AMSCs were cultured in DMEM/ITS without (A–D) or with ASE (E–H) for different times. α-SMA-expressing stress fibers were gradually decreased from Days 0 to 6 after addition of ASE (I–L), and such a change correlated well with morphological changes (E–H). Ki67 staining showed that there was no cellular proliferation no matter if ASE was added or not (M, N, respectively). AMSCs in P1 cultures showed occasional Ki67-positive nuclei (O), while many human corneal fibroblasts cultured in DMEM/10% FBS showed Ki67-positive nuclei (P). Bars represent 100 µm.