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. Author manuscript; available in PMC: 2010 Mar 16.
Published in final edited form as: J Biol Chem. 2004 Nov 22;280(5):3847–3861. doi: 10.1074/jbc.M412299200

FIG. 2. Southern Blot analyses of the L. mexicana chitinase 1 gene locus.

FIG. 2

A. Southern hybridization of L. mexicana gDNA with the LmexCht1-DIG1151 probe. gDNA (5 μg) was digested with various individual restriction endonucleases ( EcoRI, Hind III, Apa I, Not I, BamH I, Sph I and Mlu I, as indicated), separated in 1% agarose gel, transferred to a nylon membrane and hybridized with the digoxigenin-labeled LmexCht1-DIG1151probe (i.e. corresponding to nt 1 - nt 1151 of the LmexCht1-ORF). DNA standards (in bp) are shown on the left. B. Southern hybridization of the Lmex-Cos1 cosmid DNA with the LmexCht1-DIG1151 probe. Cosmid DNA (1 μg) was digested with the same restriction endonucleases as shown in Panel A and subjected to Southern hybridization with the LmexCht1-DIG1151 probe. DNA standards (in bp) are shown on the left as in Panel A.