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. Author manuscript; available in PMC: 2010 Mar 16.
Published in final edited form as: J Biol Chem. 2004 Nov 22;280(5):3847–3861. doi: 10.1074/jbc.M412299200

TABLE 1.

Soluble -released chitinase activity in L. mexicana parasite culture supernatants.

Developmental Stage Total Activity Immunoprecipitated Activityc
(pmoles of 4MU released ml−1 h−1)
Exochitinase
activitya 		Endochitinase
activityb 		Exochitinase
activitya 		Endochitinase
activityb
Promastigotes 1081 ± 102 1375 ± 142 497 ± 55 632 ± 73
Amastigotes 2440 ± 289 6207 ± 583 1122 ± 148 2731 ± 310
a

Activity assayed using 4MU-chitibiose as substrate.

b

Activity assayed using 4MU-chitotriose as substrate.

c

Activity immunoprecipitated using an anti-LdCht1-peptide antibody in a protein A-Sepharose 4B/CL bead-based assay.

Immunoprecipitated complexes were assayed for exochitinase and endochitinase activity using the appropriate substrate. The results were normalized by subtracting values obtained with NRS (i.e. preimmune serum from same rabbit). The data shown reflect the mean results of triplicate assays for each sample from three separate experiments.