TABLE 2.

Chitinase activity released by L. mexicana transfectants during short-term release assays^* in vitro.

Developmental Stage /transfected with	Total Chitinase Activitya		Immunoprecipitated Activityb
	(pmoles of 4MU released ml−1 h−1)
	LmexCht1∷HA	pKSNEO	LmexCht1∷HA	pKSNEO
Promastigotes	900 ± 123	64 ± 10	114 ± 18	-
Amastigotes	1500 ± 173	369 ± 45	183 ± 26	-

^*Parasites were harvested at mid log phase (~1.5 × 10⁷ ml⁻¹), washed and incubated at 5 × 10⁷ cells ml⁻¹ in an appropriate balanced buffered solution for 4 h and cell-free culture supernatants were assayed for activity.

^aActivity assayed using 4MU-chitotriose as substrate.

^bActivity immunoprecipitated using a mouse anti-HA monoclonal antibody Affinity Matrix (Covance). Bound-immunoprecipitates were assayed for chitinase activity using 4MU-chitotriose as substrate.