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Primer sequences for mRNA analysis by real-time polymerase chain reaction.
| Gene | Primers (human) | Amplicon | Tm |
|---|---|---|---|
| GADPH | F: 5'-cgagatccctccaaaatcaa-3'; | 170 | 60 |
| R: 5'-ttcacacccatgacgaacat-3' | |||
| MITF-M | F: 5'-ttatagtaccttctctttgccagtcc-3' | 146 | 52 |
| R: 5'-gtttatttgctaaagtggtagaaaggtact-3' | |||
| MITF-Mdel | F: 5'-ttatagtaccttctctttgccagtcc-3'; | 120 | 52 |
| R: 5'-cttataaaatccctgccgttgg-3' |
