Figure 4. Endogenous BORIS mRNA expression is induced by 5-aza-2′-deoxycytidine.

BORIS mRNA levels in MCF-7 cells were determined by qRT-PCR (as in Figure 2). Endogenous BORIS mRNA was detectable (1.02% TBP) only after treatment with the demethylating agent, 5-aza-2′-deoxycytidine (5-aza-dC) and at a level approximately one tenth of that in testis (1.02% vs. 10.47% TBP).