Figure 2. Self-renewing LSCs are enriched in the c-Kit^midCD3⁺ compartment.

a, LSC identification. The experimental design is illustrated in Supplementary Fig. 3. Top: illustration of the three subpopulations that were sorted from BM of five independent leukaemic donor mice (cell fractions are denoted on each FACS plot). Bottom: summary of independent transplantation experiments with sorted and serially diluted cells. +, leukaemia development; −, leukaemia-free for more than 100 days; n.a., viable cells after sorting were not enough for transplantation. b, LSCs are self-renewing and lead to accelerated leukaemogenesis during serial transplantations. Top: illustration of the experimental procedure. Bottom: summary of the results. Red lines in the lower chart represent the means of leukaemia latencies.