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. Author manuscript; available in PMC: 2011 Mar 16.
Published in final edited form as: Dev Cell. 2010 Feb 11;18(3):371–384. doi: 10.1016/j.devcel.2010.01.010

Figure 1. Absence of ubiquitinated H2A in the XY body did not affect meiosis in RNF8-deficient testes.

Figure 1

a.-d. Ubiquitinated H2A and ubiquitinated proteins (FK2) were absent in the XY body of RNF8-deficient spermatocytes, while γH2AX and BRCA1 were intact. Typical immunostaining images of spermatocytes at pachytene stage are shown. SCP3 represents synapsed chromosomes and γH2AX marks the XY body. e. RNA polymerase II remained excluded from the XY body in RNF8-deficient spermatocytes. Typical immunostaining images of spermatocytes at pachytene stage are shown. f. Sex chromosome inactivation was maintained in RNF8-deficient mice. Real time PCR results of expression levels of several genes on sex chromosomes in purified spermatocytes are shown. The levels in WT mice were arbitrarily set to 1 and those in RNF8-deficient mice were normalized accordingly. See also Supplementary Figure S3.