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. Author manuscript; available in PMC: 2011 Mar 16.
Published in final edited form as: Dev Cell. 2010 Feb 11;18(3):371–384. doi: 10.1016/j.devcel.2010.01.010

Figure 3. Spermiogenesis was defective in RNF8-deficient mice.

Figure 3

a. Number of sperm was decreased in RNF8-deficient mice. Sperm from 3-month old wild type (WT) and RNF8-deficient (KO) mice were counted. Sperm from both cauda epididymis were included. 6 mice of each genotype were used, p<0.001. b. Abnormal rounded sperm heads were observed in RNF8-deficient mice. Upper, typical morphologies of sperm from wild type (WT) and RNF8-deficient (KO) mice with hematoxylin staining are shown; Lower, quantification of sperm with abnormal rounded head is shown. c. Majorities of sperm from RNF8-deficient mice were immotile. Sperm mobility of wild type (WT) and RNF8-deficient (KO) mice are compared. d. H&E staining of paraffin sections of cauda epididymis from wild type (WT) and RNF8-deficient (KO) mice are shown. On right are images with higher magnification showing details of sperm heads. e. Sperm were less condensed and contained residue cytoplasm. Typical electron microscopic images of sperm heads from wild type (WT) and RNF8-deficient (KO) are shown. See also Supplementary Figure S5.