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. Author manuscript; available in PMC: 2011 Mar 16.
Published in final edited form as: Dev Cell. 2010 Feb 11;18(3):371–384. doi: 10.1016/j.devcel.2010.01.010

Figure 4. Histones failed to be replaced in sperm from RNF8-deficient mice.

Figure 4

a. Protamines failed to replace histones in RNF8-deficient sperm. Typical immunostaining images of sperm from wild type (WT) and RNF8-deficient (KO) mice are shown. Antibodies used are indicated. Prm1: protamine 1; Prm2: protamine 2. b. mRNA levels of transition proteins and protamines were similar in wild type and RNF8-deficient mice. Semi-quantitative RT-PCR was performed using testes cDNA from wild type (WT) and RNF8-deficient (KO) mice. Tnp1: transition protein 1; Tnp2: transition protein 2. GAPDH was used as internal control. c. Incorporation of transition proteins into chromatin was defective in RNF8-deficient mice. Western blots using testes from wild type (WT) and RNF8-deficient (KO) mice are shown. Both NETN-soluble and HCl-released chromatin-bound fractions were used. Antibodies used are indicated, and Plk1 and H3K27me3 were used as soluble and chromatin-bound fraction loading controls respectively. See also Supplementary Figure S6.