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. Author manuscript; available in PMC: 2011 Mar 16.
Published in final edited form as: Dev Cell. 2010 Feb 11;18(3):371–384. doi: 10.1016/j.devcel.2010.01.010

Figure 5. Ubiquitinated H2A, H2B and H4 K16 acetylation were decreased in RNF8-deficient testes.

Figure 5

a.-d. ub-H2A, ub-H2B and K4 K16 acetylation were decreased in elongating spermatids of RNF8-deficient mice. Typical immunostaining images of testes seminiferous tubules at similar stages from wild type (WT) and RNF8-deficient (KO) mice are shown. SC: spermatocytes; ES: elongating spermatids. Antibodies used are indicated, FK2: antimono/poly-ubiquitinated proteins. e.-h. Typical immunostaining images of individual elongating spermatids from wild type (WT) and RNF8-deficient (KO) mice are shown. i. ub-H2A, ub-H2B and H4 K16 acetylation were decreased in testes from RNF8-deficient mice. Western blots of chromatin-bound proteins using testes from wild type (WT) and RNF8-deficient (KO) mice are shown. Antibodies used are indicated, and H4 was used as loading control.