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. Author manuscript; available in PMC: 2011 Mar 16.
Published in final edited form as: Dev Cell. 2010 Feb 11;18(3):371–384. doi: 10.1016/j.devcel.2010.01.010

Figure 6. RNF8 controlled chromatin association of H4K16 acetyltransferase MOF.

Figure 6

a. ub-H2A, ub-H2B and H4 K16 acetylation were decreased in RNF8-deficient MEFs. Western blots of chromatin-bound proteins from wild type (WT) and RNF8-deficient (KO) MEFs are shown. Antibodies used are indicated, and H4 was used as loading control. b. Depletion of RNF8 affects not only H2A and H2B ubiquitination, but also H4K16 acetylation. Western blots of chromatin-bound proteins from control siRNA or RNF8 siRNA-treated HeLa cells are shown. c. MOF is enriched in elongating spermatids. Typical immunostaining images of seminiferous tubules at similar stages from wild type (WT) and RNF8-deficient (KO) mice are shown. SC: spermatocytes; ES: elongating spermatids. Antibodies used are indicated. d.-e. Chromatin-associated MOF was decreased in both testes and MEFs from RNF8-defiecient mice. Western blot using wild type (WT) and RNF8-deficient (KO) testes (d) and MEFs (e) are shown. Both NETN-soluble and chromatin-bound fractions were used. Antibodies used are indicated. Plk1 and H3K27me3 were used as soluble and chromatin-bound fraction loading controls, respectively. f. Depletion of MOF affects H4K16 acetylation only, but not H2A and H2B ubiquitination. Western blots of chromatin-bound proteins from control siRNA or MOF siRNA-treated HeLa cells are shown.