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. Author manuscript; available in PMC: 2011 Mar 31.
Published in final edited form as: Vision Res. 2009 Sep 3;50(7):686–697. doi: 10.1016/j.visres.2009.08.028

Figure 4. Transduction Properties of POD-GFP following intravitreal injection in vivo.

Figure 4

POD-GFP or GFP protein was injected into the intravitreal space of adult mice and eyes harvested after 6 hours. POD-GFP was detected (a) in the ganglion cells and a subset of cells in the inner nuclear layer (INL) (arrowhead) and dendrites in the inner retina (arrow). Significant GFP-associated fluorescence could not be detected above background auto fluorescence associated with uninjected or GFP-injected eyes (b). Total GFP intensity associated with each experiment is quantified in (c). Apparent GFP-signal in uninjected eyes is auto fluorescence of the outer segments (OS), which was not typically observed in GFP-injected eyes. Significant binding of POD-GFP to the lens capsule was noted (d). ONL, Outer nuclear Layer; INL, Inner Nuclear Layer; GCL, Ganglion Cell Layer; RPE, Retinal Pigment Epithelium. Uninjected, n = 3, GFP and POD-GFP, n = 4. Color version of this figure appears online.