Western blots were performed using the cell lysates derived from U87MG-NT cells infected with a mixture lentiviruses carrying non-targeting shRNAs (A and C), and U87MGshRNA-CD44 cells infected with a mixture of lentiviruses carrying CD44-specific shRNAs (B and D). These cells were treated with or without 60µm H2O2 for 30min, 2h, 24h, 48h, and 72h as indicated in the panels. 100µg of total protein were loaded in each lane. Actin was included as an internal control for protein loading. The antibodies used against the different signaling mediators are indicated in the panels.