Fig. 4.

PLL dendrimer reduces CD31 + cells and enhances apoptosis in tumors after i.v. administration. Murine B16F10 melanoma tumors were implanted s.c. PLL dendrimer (50 mg/kg/day) or PBS alone were injected i.v. twice on day 1 and 2 after tumor inoculation. Tumors were excised on day 10 and stained for CD31 or TUNEL. (A) Number of CD31 + cells per region of interest. Data were expressed as a mean ± SD from 12 different fields of view (^∗∗∗P < 0.001). (B) Tumor sections H&E stained (top row) or TUNEL and propidium iodide nuclear counterstained to identify apoptotic (green) cells from the total cell population (red) in the bottom row.