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Function of A333G mtPheRS in E. coli. Rescue of the growth phenotype of E. coli NP37 transformed with (A) yeast MSF1, msf1-1, or msf1 with a stop codon at A333 and (B) human FARS2, fars2-1, or fars2 with a stop codon at A308. (C) Transediting activity of E. coli wild-type and NP37 FARS2 complemented cell extracts grown at 30 or 42 °C. Reactions were carried out at 42 °C. Data points are an average of three independent experiments with errors bars representing 1 SD.
