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. 2010 Feb 16;107(9):4063–4068. doi: 10.1073/pnas.0909640107

Fig. 4.

Fig. 4.

Function of βD243A ctPheRS in S. cerevisiae. (A) Dissection of tetrads from S. cerevisiae FRS1/frsΔ complemented with pFL36-FRS1, pFL36-frs1-1, or pFL36 onto YPD and replica plated onto G418 and CSM-Leu. In liquid YPD doubling times for frsΔ pFL36-FRS1 and pFL36-frs1-1 were 1.37 ± 0.05 and 1.34 ± 0.09 h, respectively. (B) Posttransfer editing activity of S. cerevisiae frs1Δ pFL36-FRS1 and frs1Δ pFL36-frs1-1 extracts at 37 °C. Data points are an average of at least three independent experiments, with errors bars representing 1 SD.