Table 1.
Hemolytic activity of CDC derivatives containing mutations in the cholesterol-binding motif
| Toxin | HD50 (nM) | % WT activity |
| PFO | 0.34 ± 0.1 | 100 |
| PFOT490A•L491A | >1410 | <0.03 |
| PFOT490G•L491G | >1760 | <0.02 |
| SLO | 0.8 ± 0.1 | 100 |
| SLOT564G•L565G | >1640 | < 0.02 |
| PLY | 0.7 ± 0.3 | 100 |
| PLYT459G•L460G | >1890 | <0.02 |
| PFOT490S | 3.2 ± 0.7 | 11 |
| PFOL491I | 1.5 ± 0.6 | 22 |
| PFOL491V | 1.9 ± 0.1 | 18 |
| PFOT490S•L491I | 83 ± 6 | 0.4 |
| PFOT490S•L491V | 92 ± 2 | 0.3 |
| PFOT490L•L491T | >1760 | <0.02 |
Shown are the HD50 values for PFO, SLO, and PLY and their derivatives containing mutants in the cholesterol recognition motif.