Fig. 1.

Assessment of constitutive activity in IP production of 14 naturally occurring mutations of the hGnRHR after pharmacoperone rescue. COS-7 cells were transfected with 100 ng of WT or mutant cDNA as described in Materials and Methods. Mutants were incubated in media alone (shown as two dashed lines showing upper and lower level of IP production) or rescued with pharmacoperone (In3) as described in Materials and Methods; In3 was then washed out and IP production was measured in response to media alone (no agonist added) (Upper) or rescued with pharmacoperone (In3), then In3 was washed out and agonist added. In all figures, SEMs are shown for least three independent experiments performed in replicates of 4–6 (Lower).