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Purification of porcine daintain/AIF1. (A) HPLC on Pharmacia-LKB TSK ODS 120-T, the active fraction is indicated by a bar. (B) Additional HPLC on Vydac 218TP54, the main peak indicated by a bar corresponds to daintain/AIF1. (C) Two micrograms of the purified sample applied to capillary zone electrophoresis at 25 kV, 25°C in 50 mM phosphate, pH 2.5, showing a symmetrical peak.
