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. 2010 Feb 10;107(9):4093–4098. doi: 10.1073/pnas.0914612107

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Fig. 3. — Differences in DNA–Scr-HD interactions between different Scr-HD variants visualized in polytene nuclei of live salivary gland cells by APD imaging and FCS. (A–D) Polytene nuclei expressing the synthetic Scr-HD_wt, Scr-HD_AA, Scr-HD_DD and at low, physiologically relevant concentrations [Scr-HD_wt] = 105 nM, [Scr-HD_AA] = 157 nM, [Scr-HD_DD] = 66 nM and . (E–F) Differences in DNA–Scr-HD interactions between the transcriptionally active and inactive variants can be readily observed at low but are less distinguishable at high Scr-HD concentrations.

Inline graphic — Differences in DNA–Scr-HD interactions between different Scr-HD variants visualized in polytene nuclei of live salivary gland cells by APD imaging and FCS. (A–D) Polytene nuclei expressing the synthetic Scr-HD_wt, Scr-HD_AA, Scr-HD_DD and at low, physiologically relevant concentrations [Scr-HD_wt] = 105 nM, [Scr-HD_AA] = 157 nM, [Scr-HD_DD] = 66 nM and . (E–F) Differences in DNA–Scr-HD interactions between the transcriptionally active and inactive variants can be readily observed at low but are less distinguishable at high Scr-HD concentrations.