Fig. 2.
Expression of Hydra myc1 and max genes and their protein products. (A) Northern analyses using aliquots (2.0 μg) of poly(A)+-selected RNAs from whole Hydra animals and Hydra myc1 or max specific cDNA probes. Each filter was stripped and rehybridized with a Hydra CAD specific cDNA probe. (B) Immunoprecipitation analysis using aliquots (5 × 106 cpm) of boiled cell extracts from [35S]methionine-labeled Hydra animals and a polyclonal antiserum directed against Hydra Myc1 recombinant protein (α-hy Myc1), or normal rabbit serum (NRS). For comparison, [35S]methionine-labeled Hydra Myc1 p39 and p36 proteins were also produced by in vitro translation of corresponding cDNAs cloned in Bluescript (BS) vectors and immunoprecipitated. Proteins were analyzed by SDS/PAGE (10%, wt/vol). (C) Immunoblot analysis of a 30-μg aliquot of total cell proteins from whole Hydra polyps and of in vitro translated Hydra Max using a polyclonal antiserum directed against Hydra Max recombinant protein (α-hy Max). Proteins were resolved by SDS/PAGE (12.5%, wt/vol).