Fig. 2.

Comparing free ACPP and ACPPD for fluorescence imaging guidance. White light (A and D) and fluorescence images showing GFP-labeled MDA-MB 435 xenografts (B and E) from mice that were treated with Cy5-labeled free ACPP (C) and ACPPD (F). In tumors of comparable size, treatment with the free ACPP (C) or ACPPD (F) resulted in tumor-specific fluorescence uptake. There is a higher tumor-to-background fluorescence contrast for the ACPPD compared to free ACPPs and a higher absolute tumor fluorescence for ACPPDs when normalized to Cy5 fluorescence injected. Cy5 fluorescence images (C and F) were adjusted for total nanomoles of Cy5 injected per animal.