Fig. 2.

In vitro effects of GW9662 and BIO on Wnt signaling in hMSCs. (A) BIO and GW are predicted to up-regulate Wnt signaling by direct inhibition of GSK3β or by ablating PPARγ-mediated negative crosstalk, respectively. (B) Fluorescent microscopy of hMSCs with counterstained nuclei (pico-green; Left) or cy-3 labeled anti-β-catenin stained nuclei (Right) (C) Immunoblots of cytosolic GSK3β (C GSK3β), nuclear β-catenin (N β-cat), and nuclear PPARγ (N −PPARγ) on extracts of hMSCs treated with BIO or GW. Blots were normalized for cytosolic proteins with GAPDH (C GAPDH) and for nuclear proteins by silver stain. (D) Immunoblots of whole cell and cytosolic extracts of hMSCs.