Fig. 1.
Thyroid status alters Mc4r expression in the hypothalamic PVN. (A) Real-time PCR quantification of Mc4r and Trh mRNA from PVN of adult male mice treated for 13 days by PTU (hypothyroid) or T4 (hyperthyroid), or euthyroid controls (Ct). Gene expression was normalized with Gapdh. Shown are pooled results of two independent experiments (n = 7 or 8 per group). Nonparametric ANOVA followed by permutation test was used to assess statistical significance. **, P < 0.01; ***, P < 0.001. (B) In situ hybridization shows a decrease in Mc4r mRNA expression in the PVN following T4 treatment, compared with PTU-treated mice. pvn, paraventricular nuclei; VIII, third ventricle. (Scale bar, 1 mm.)