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Analysis of the autophagic effect on HBV RNAs. Huh7.5 cells were transfected with pUC19 or the 1.3mer HBV genome and treated with 3-MA or siRNA as described in the legend to Fig. 3. (A and B) Northern blot analysis of HBV RNAs. (C and D) Primer extension analysis of total HBV precore and core RNAs in cells (lanes 1–3) or the core RNA packaged in core particles (lanes 4–6). S, S gene transcripts; C, C gene transcripts.
