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. 2010 Feb 16;107(9):4105–4110. doi: 10.1073/pnas.0907072107

Fig. 2.

Fig. 2.

Evidence of RNA looping by PTB34. (A) Fluorescence emission spectra of labeled PPT-15 RNA in the absence (blue) and presence of 100 nM PTB34 (red). (B) FRET ratios for PPT-5 through PPT-30 in the absence (blue) and presence of 100 nM PTB34 (red). (C) FRET ratio of PPT-15 as a function of [PTB34]. The black line is a fit to a modified Hill equation (Methods). Error bars stem from three independent assays. (D) Apparent KD as a function of the intervening loop size. Error bars stem from the standard deviation to the fit as shown in C.