Table 1.
Melanin | Ddc | ebony | |||||||
Effect | df | F | P | df | F | P | df | F | P |
Clone | 48 | 1.21 | 0.199 | 20 | 2.36 | 0.026 | 20 | 1.44 | 0.203 |
Population | 2 | 15.37 | 0.000 | 2 | 1.35 | 0.281 | 2 | 0.97 | 0.395 |
Fish | 1 | 180.46 | 0.000 | 1 | 0.07 | 0.798 | 1 | 9.44 | 0.006 |
UV | 1 | 92.31 | 0.000 | 1 | 3.00 | 0.097 | 1 | 0.09 | 0.767 |
UV x fish | 1 | 27.72 | 0.000 | 1 | 13.19 | 0.001 | 1 | 0.25 | 0.621 |
Each response variable was modeled as a function of clone nested within population, population nested within fish history, fish history, UV treatment, and fish history by UV treatment interaction, with clone as a random effect. Significant results (P < 0.05) are indicated in bold. Melanin data include replicates within clones; the error df = 121 and total df = 174. For gene expression, error df = 22 and total df = 47.