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General linear model results
| Effect | Coefficient | df | F | P |
| Pop | 2 | 9.99 | 0.000 | |
| Fish | −0.22 | 1 | 86.09 | 0.000 |
| UV | 0.12 | 1 | 28.14 | 0.000 |
| ebony | −1.31 | 1 | 9.30 | 0.004 |
| Ddc | −1.66 | 1 | 12.20 | 0.001 |
| Ddc x ebony | 3.02 | 1 | 8.54 | 0.006 |
Melanin is modeled as a function of population nested within fish history, fish history, UV treatment, and expression of ebony, Ddc, and ebony by Ddc interaction. Significant results (P < 0.05) are indicated in bold. The error df = 39 and total df = 46.
